Regulatory

Part:BBa_K1933004:Design

Designed by: Tomoki Uchino   Group: iGEM16_Kyoto   (2016-10-10)


constitutive promoter and RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Nonsense sequence (7 bp) was added after promoter to enhance translation efficiency.

Source

  • BBa_J23105 was designed by iGEM2006_Berkeley
  • BBa_B0034 was designed by Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
    This part was chemically synthesized.